(center map) (center map)logical re631_s3146_re638=FALSE PMID:9381178 in FL5.12 (murine pro-B-cell lymphoid cell, IL-3 dependent) BAD phosphorylated upon IL-3 in 293T, over-expression of wt Akt or myr-Akt, but not kinase mutant induces phosphorylation of BAD in vitro phosphorylation of BAD by Akt, BAD S112(human S75)A-S136(human S99)A not phosphorylated PMID:8929531 2D tryptic peptide mapping identifies S112(human S75) and S136(human S99) as the phospho-sites phsophorylation in FL5.12 in response to IL-3 or PMA PMID:9346240 in vitro, purified Akt phosphorylates recombinant BAD at S136(human S99) but not at S112(human S75) (serine mutants) in Balb/c 3T3 upon PDGF or PC12 upon NGF, BAD phosphorylated in an Akt-dependent manner (wortmannin, LY294002) partial evidence for BAD Akt interaction PMID:10230394 mitochondria localized PKA is a BAD-S112(human S75) kinase PMID:10558990 in vitro purified activated RSK2/RPS6KA3 phosphorylates a peptide encompassing BAD S112(humanS75) phospho-site. Far less efficient as a BAD-S136 kinase in 293T, over-expression of RSK2 and MEK1 induces phsophorylation of BAD (anti-BAD pS112) in BDNF-treated cerebellar granule cells, BDNF induces S112 phosphorylation of BAD, in a MEK1-dependent manner (PD098059) PMID:10837486 RSK is a BAD S112 kinase (anti BAD-phosphoS112) PMID:10837486 in vitro, in HEK293 upon forskolin TPA isoproterenol or insulin-like growth factor, PKA mediated phosphorylation of S155 of murine BAD (human S118) S118 lies in the BH3 domain of BAD phosphorylation of S155 independent of that of S112/136 RSK1-2-3 are BAD S155 kinases PMID:12954615 in vitro, murine PIM2 immunoprecipitated from trasfected U2OS phosphorylates BAD in U2OS, PIM2 expression but not that of a kinase dead mutant induces BAD S112 phosphorylation in FDCP1 murine IL3 dependent hematopoietic cells, upon IL3 withdrawal or doxorubicin, PIM2 ectopic expression is protective PMID:15280015 BAD S112 phosphorylation by PIM1 PMID:17270021 in SW480 human colon carcinoma cell line, correlation between PIM3 expression and BAD phosphorylation. siRNA against PIM3 abrogates BAD S112 and S136 phosphorylation and induces apoptosis in a set of human colon cancers, correlation and colocalization of PIM3 and BAD phospho-S112 PMID:11493700 S6K purified as a BAD kinase from FL5.12 BCL-XL/BAD cells in vitro with recombinant proteins, BAD S136 phosphorylation in FL5.12 by S6K upon IGF, inhibited by rapamycin in ES p70S6K-/- cells, IGF-induced BAD S136 phosphorylation is lost PMID:10611223 in FL5.12 upon IL3, PAK1 phosphorylates BAD on S112 and S136, which results in decreased interaction with BCL2 and BCL2L1, and increased interaction with 14-3-3