(center map) (center map)PMID:15989957 in Hela MCL1 ubiquitination mainly on lysines 136, 194, and 197 purification of MCL1 E3 ubiquitin ligase from the cytosol of Hela cells in vitro reconstitution of the system HUWE1 KD PMID:16543145 suggest that HUWE1 preferentially target S159 phosphorylated MCL1 for polyubiquitination PMID:16213503 HUWE1 is a BH3 domain protein - competition HUWE1 / Bim for binding to MCL1