SMETHILLIUM: Spatial normalisation METHod for ILLumina InfinIUM HumanMethylation BeadChip
DNA methylation is a major epigenetic modification in human cells. Illumina HumanMethylation27 BeadChip investigates the methylation of 27,578 loci in the genome. We developed a normalisation method to correct the spatial background noise which affect the signal-to-noise ratio. The prediction performance of the proposed method was assessed on methylated and unmethylated samples. We demonstrate that the spatial normalisation outperforms BeadStudio to predict the methylation status of a given locus
A R script which performs the spatial normalisation is available.